Journal: Neuron

Article Title: BAD-Dependent Regulation of Fuel Metabolism and K ATP Channel Activity Confers Resistance to Epileptic Seizures

doi: 10.1016/j.neuron.2012.03.032

Figure Lengend Snippet: (A and B) Raw seizure scores in 8–10-wk old male Bad−/− (A) and Bad S155A (B) mice compared with wild-type control animals over a 4-hour period after a single i.p. injection of kainic acid (KA) at 30 mg/kg. See also Figure S2. (C) Integrated seizure severity of the above experimental cohorts and Bid−/− mice similarly treated with KA. The scoring scale and the formula used to derive seizure severity are provided. Wild-type, n=42; Bad−/−, n=13; Bad S155A, n=20; Bid−/−; n=9. (D) Integrated seizure severity in Bad−/− and wild-type mice (n=16) subjected to a single s.c. injection of pentylenetetrazole (PTZ) at 80 mg/kg monitored over a 70 minute-period. See also Figures S3 and S4. Data in A through D are presented as mean ± SEM. *** p < 0.001; n.s., non-significant; two-tailed Student’s t-test.

Article Snippet: Kainic Acid Treatment and Monitoring of Behavioral Seizures Kainic acid (KA) (Tocris Bioscience, MO, US) was dissolved in saline (Sigma) and injected intraperitoneally (i.p.) at a dose of 30 mg/kg of body weight.

Techniques: Control, Injection, Two Tailed Test

Journal: Neuron

Article Title: BAD-Dependent Regulation of Fuel Metabolism and K ATP Channel Activity Confers Resistance to Epileptic Seizures

doi: 10.1016/j.neuron.2012.03.032

Figure Lengend Snippet: (A) A single representative EEG channel recording of approximately 1 hour for each genotype (right frontoparietal electrode). A single i.p. injection of kainic acid (30 mg/kg of body weight) was administered at the indicated time. The green block arrow near the end of the wild-type recording indicates the time of death during generalized tonic-clonic status epilepticus. (B) The average fraction of time spent in a high energy spiking period for each genotype during a 30 minute period beginning 3 minutes after injection. EEGs were scored by an investigator blind to the genotype, guided by both the raw EEG traces and a trace of power in the 20–70 Hz band to identify onset and offset of high energy spiking (typically seen as rapid increases or decreases of ≥5 dB in power). Wild-type, n=17; Bad−/−, n=20; mean ± SEM, * p < 0.05 by two-tailed Student’s t-test. In these cohorts, 9 of 17 wild-type and 0 of 20 Bad−/− animals died during status epilepticus.

Article Snippet: Kainic Acid Treatment and Monitoring of Behavioral Seizures Kainic acid (KA) (Tocris Bioscience, MO, US) was dissolved in saline (Sigma) and injected intraperitoneally (i.p.) at a dose of 30 mg/kg of body weight.

Techniques: Injection, Blocking Assay, Two Tailed Test

Journal: Neuron

Article Title: BAD-Dependent Regulation of Fuel Metabolism and K ATP Channel Activity Confers Resistance to Epileptic Seizures

doi: 10.1016/j.neuron.2012.03.032

Figure Lengend Snippet: (A and B) Raw scores (A) and seizure severity (B) in Bad−/− and Kir6.2−/− single mutants and Bad−/−; Kir6.2−/− double mutant mice compared with wild-type mice after a single i.p. injection of kainic acid monitored and analyzed as in Figure 3. Data are presented as mean ± SEM. ** p < 0.01; *** p < 0.001; n.s., non-significant; two-tailed Student’s t-test.

Article Snippet: Kainic Acid Treatment and Monitoring of Behavioral Seizures Kainic acid (KA) (Tocris Bioscience, MO, US) was dissolved in saline (Sigma) and injected intraperitoneally (i.p.) at a dose of 30 mg/kg of body weight.

Techniques: Mutagenesis, Injection, Two Tailed Test